Does anibody knows if the Anti-beta Amyloid 1-42 antibody reacts with extracleular peptyde or only with intracelular?

More Marielza Andrade Nunes's questions See All

How to break TPP-chitosan NPs? How to avoid aggreation?

Hey, hi, I am preparing Chitosan-TPP NPs in the ratio 1 (TPP) : 5 (CHI), I am encapsulating DNA inside the NPs and to measure the E.E. I am spinning down the particles and measuring the...

26 October 2020 9,898 3 View

How should one treat raw data from Zetasizer (Nano ZS) Analysis?

Greetings all, I'm interested in studying the zeta size of a couple of nanoparticles solutions I have tested on simple acute toxicity assays. Those NPs were suspended in three or four different...

30 September 2020 9,243 7 View

What are the biggest challenges for solid-state batteries?

I was reading that there was a strong interest in the development of solid-state batteries and solid electrolytes (SEs). However, a limitation reported in several studies is the solid-solid...

03 June 2020 7,635 7 View

What are the new potential materials to use in a solar cell?

Are there prospects for new materials that can increase the energy efficiency of cells? Will these materials have similar durability to silicon cells?

07 May 2020 3,769 18 View

Could the Cd assume values greater than 1?

I am developing a regression model to predict the mass flow rate of a Direct Expansion Solar Assited CO2 heat pump (for a needle valve expansion device). The mass flow is based on the following...

12 February 2020 5,757 3 View

Does anybody have access to these papers?

Churchill, Stuart W., and H. Ozoe. "Correlations for laminar forced convection with uniform heating in flow over a plate and in developing and fully developed flow in a tube." journal of Heat...

06 February 2019 7,397 3 View

How can I prevent glycerol adsorption by PDMS after a couple of days in the incubator?

Cheers everyone I'm currently working with a microfabricated device composed by a thin PDMS sheet assembled onto a glycerol-coated glass slide. I need the PDMS to glide over the glass, hence I'm...

19 September 2018 4,705 3 View

Is CMV a strong promoter for mouse embryonic fibroblasts?

We've attempted PEI transfection as well as lentiviral transduction to over express a tagged protein under a CMV promoter. The plasmid is highly expressed in Hek293T cells but not in the MEFs.

06 August 2018 5,724 3 View

Can I use Support Vector Regression (SVR) in multivariate data analysis?

I'm trying to perform a model to correlate reflectance spectra (each spectra has 40 wavelenghts) x respmonse variable. Do SVR a good method to do that?

05 May 2018 2,107 3 View

No title

How can I use Machine learning Support Vector Machine (SVM) in regression problems?

27 April 2018 3,244 24 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Holger Wille

Good monoclonal antibodies will react with both, extracellular and intracellular peptides. It is more the fixation procedure that can obscure epitopes in one or the other location.

Bongmun Kang

I think the number and purity of antibody can affect the reaction

Marielza Andrade Nunes

Thanks for the answers. It was very helpfull.