Does anibody knows if the Anti-beta Amyloid 1-42 antibody reacts with extracleular peptyde or only with intracelular?

More Marielza Andrade Nunes's questions See All

Managing the waste of raw materials in industry reduces the extraction of natural resources. Can this practice be related to ESG?

My question is whether the company could be ESG with this practice alone.

10 April 2024 1,870 2 View

Can i leave a starter bacterial culture at 4ºC in its original liquid culture?

Im planning to do some BAC maxi preps, a total of 8, and due to the restrictions in terms of equipment, i would only be able to do 2 at a time since only two 1000mL Erlenmeyers fit in the shaking...

11 February 2024 3,741 5 View

Relationship between the Austrian theory of economic cycles and the bullwhip effect?

Is there any relationship between the Austrian theory of business cycles and the bullwhip effect in the supply chain?

05 January 2024 1,754 1 View

Could you identify these freshwater diatoms?

Hi everyone. I would really appreciate a second opinion about these freshwater diatoms, at least about their genera. Any help is extremely appreciated! Images are numbered and there's a scale bar...

15 July 2023 7,103 3 View

Could you give your opinion about what these freshwater cyanobacteria could be?

Hi everyone. I would really appreciate a second opinion about these freshwater cyanobacteria, at least about their genera. Any help is extremely appreciated! Thanks in advance!

15 July 2023 9,859 3 View

Good references on recommender system development?

Hello, Could anyone recommend me good references on recommender system development?

25 April 2023 4,510 4 View

What are the most relevant points in strategic planning, aiming at employability among middle-aged people in an interior city?

The city's main economic activity is tourism. Population of approximately 87 700 inhabitants The main employment activities are informal; The largest portion of the population active in the labor...

24 April 2023 1,678 0 View

Does anyone have tips on technologies that can capture images and voice of pets?

I'm starting a research on technologies that can capture images and voice of pets, according to your behavior. Can anyone give me tips for articles or tools that help me in this study?

23 April 2023 8,190 2 View

Are these structures heterocysts in these true-branched filamentous cyanobacteria?

Hi. I'm having some trouble in identifying heterocysts in my samples of true-branched filamentous cyanobacteria, mostly Stigonema. Are these structures pointed by the red lines heterocysts? I...

30 December 2022 1,564 7 View

Is there any good reference examples about Metaprogramming in Ruby?

Dear all, We are current doing a research which involves developing an internal DSL using metaprogramming in Ruby. It's not a clear topic to us and I would appreciate any good references/examples...

16 November 2022 659 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Pragmatic inquiry research design?

Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...

28 July 2024 540 2 View

Holger Wille

Good monoclonal antibodies will react with both, extracellular and intracellular peptides. It is more the fixation procedure that can obscure epitopes in one or the other location.

Bongmun Kang

I think the number and purity of antibody can affect the reaction

Marielza Andrade Nunes

Thanks for the answers. It was very helpfull.