How to design specific primer to detect resistace gene on isolated plasmid?

05 December 2024 1 1K Report

I isolated pathogenic bacteria from cancer patients, I will isolate plasmid DNA from these bacteria. I want to design suitable primers to detect ESBLs on plasmid, How and What is the suitable tool to design primers?

Katie A S Burnette

What do you already know about these bacteria and their plasmids? Check the literature to see if someone else has designed a similar assay.

Also, not sure if you need to design a PCR-based assay or not. Another option is to pay to have the entire plasmid sequenced.

Hope that helps!

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