For flow cytometric analysis of N1 and N2 phenotype of TANs in breast tumor mice, I need to specifically identify them for ordering antibody and is unable to confirm the correct one. If anybody knows please help.
We have been getting this black dots in our cell culture. But it is not affecting pH of media (no color change observed) until 4 or 5 days. Cells are attaining morphology within 24hrs but once the...
12 May 2024 9,284 4 View
I need the steps to add the LCR instrument to the LabVIEW program, and I already installed the software in the system but am unable to identify the instrument.
12 March 2024 2,968 0 View
i have been performing quantitative phytochemical test for flavonoid detection of my plant sample for past 4 days. i have been using the 10-50 concentrations of sample and still not getting any...
26 January 2024 9,301 1 View
i have been performing quantitative phytochemical analysis for past week and have found it really difficult to understand how to set a specific concentration of sample for conducting the...
26 January 2024 687 3 View
The amount of reagents to be taken
02 December 2023 3,481 0 View
procedure of preparation
19 November 2023 4,484 1 View
I think it means the biomarker responses, recently I read that there is mussel, fish and amphibian alarm systems. I need to clarify my confusions
23 June 2023 1,291 0 View
preparation method is as follows: Grinded CuO+MoO3 for 30 minutes and made pellets(pressure 6-8 ton). Sintered at 650 degree C for 12 Hrs. It is showing brown color only. I am expecting green...
08 June 2023 1,395 0 View
which column represents the acceleration data in this Excel sheet?
02 February 2023 3,237 0 View
How to get damage matrix for seismic vulnerability assessment?
30 January 2023 2,815 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View