I am running EMSAs with different DNA probes (ss/ds/stem-loop etc.). I hybridized and purified the probes, and consistently see my hybridized and PAGE-purified stem-loop probes run faster than my ssDNA probes. Is this normal?
سلام عليكم سؤال هل يشترط عدد الباحثين( ثلاث او اقل ) في اي بحث علمي في مجلة علمية للترقية العلمية اذا كنت الباحث الرابع في البحث العلمي كم نقطة احصل...
31 May 2024 6,120 3 View
When I study the density of states (DOS) of a specific nanoribbon material (e.g., graphene nanoribbon) with fixed width and length, I see that the plot is between energy and DOS, with energy on...
22 May 2024 2,044 0 View
hello Can any one identification this insect best wishes
21 May 2024 837 8 View
For easily able to understand I uploaded a image.
15 May 2024 9,768 2 View
Dear Sir/Madam, I hope that this email finds you well. My name on the paper entitled "Fertility Table and Thermal Requirements for the Development of Callosobruchus maculatus (F) Reared on...
27 April 2024 2,501 1 View
hi please any one id this beetle best wishes.
18 April 2024 7,246 5 View
hi The problem did not appear in the fifth month, but rather appeared in the third and fourth months, and the adult females remained alive for more than five days, contrary to what they say, that...
11 April 2024 2,853 0 View
Is this plant bug (Liorhysus hyalinus) a predator, a parasite, or is it of no significant economic importance?
29 March 2024 4,326 0 View
I have been invited to Peer review on Qeios. Do you recommend me to accept? www.qeios.com Thanks for the feedback @ feedback, @ Peer review
14 March 2024 6,690 4 View
Hi
26 February 2024 8,700 0 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy
02 August 2024 3,104 0 View
Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...
01 August 2024 1,168 4 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View