I am running SDS page gels using the mini-PROTEAN tetra vertical tank. Gels ran normally up until about 3/4 of the way down the gel. At this point, the dye changes from blue to yellow and became distort. I have inspected the module so this is not the problem. The electrophoresis buffer used has the correct pH. The loading dye was Laemmli Loading Buffer. All gels were run at 140V for 50-60 minutes.
I think your protein samples contain a large amount of something else that is causing interference with electrophoresis. I suspect it may be lipids or detergent, judging by the appearance of the stained gel below the bands and the drab yellow color.
You may have to clean up the samples before electrophoresis to remove this stuff.
Hi Clara,
the color change of the dye from blue to yellow indicates an acidic pH. I would think that there was something wrong with the casting projedure of your gradient gels. Maybe you order a new batch and if the problem is not happening with the new gels ask BioRad to provide a charge free replacement. Remember that the pH values of the gels needs to be appropriate for the chosen buffer system. At very low pH SDS will not bind to your proteins and the required charge (coming from the SDS) is missing on your proteins.
Best,
Murat
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