Hello everyone,
I would like to know if anyone has experience in performing RNAseq from lymphocyte suspension (Sorter, MACs, etc.) from fixed and permeabilized samples?
I was thinking of sorting T cells, stained intracellularly with IC fixation kit (BD Biosciences) and sequencing for bulk RNAseq. Since surface markers are commonly used for this, I don't know if it is possible to use intracellular staining since cells die during fixation.
Thanks