Transfected cells died after trypsinization ?

Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...

11 December 2019 3,243 5 View

Constitutive expression of eGFP in transfected cells?

Hi, I am trying to culture a stable clones using transfected cells. As the clones grow, I noticed the eGFP signal diminishes over time but the cells are still resistant to antibiotics and do not...

11 December 2019 176 14 View

New in lentivirus transduction work?

Hi all, I am new in lentivirus transduction work and have some problem as listed below: 1) Is CMV promoter considered a strong promoter to express the gene I want in the transgenic organism...

09 October 2019 8,592 4 View

Problem with stable cell line generation?

Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...

08 September 2019 9,024 2 View

Will miRNA transfection affect cell morphology?

Hi Scientist, I am transfecting an oncogenic miRNA into a breast cancer cell line and based on your experience in the mammalian cell line, will such transfection affect the cell morphology?

06 July 2019 7,378 8 View

Suggestion for good plasmid extraction kit?

Hi, Can anyone recommend a plasmid extraction kit that could produce good plasmid yield (>300ng/uL). The plasmid I am using now is a high copy number plasmid and the bacteria I am using is...

06 July 2019 6,820 3 View

MiRNA primer sequence?

Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...

05 June 2019 5,879 7 View

Co-transfection of miRNA mimics and inhibitors?

Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.

05 June 2019 9,712 3 View

Use of fluorescence or luciferase approach in checking transfection efficiency?

Hi all, I would like to seek for your opinions on whether fluorescence approach (GFP) or luciferase approach is better in assessing transfection efficiency / success (after miRNA...

03 April 2019 1,383 6 View

Single plasmid that carries both miRNA and reporter gene like GFP/luciferase?

Hi researcher, I wonder any of you have tried to design a single plasmid that carries both miRNA and reporter gene like GFP / luciferase? I am thinking with such plasmid, I will just need to...

03 April 2019 5,144 7 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

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01 March 2021 3,905 5 View

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Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View