Hi all,
I would like to ask what is the best way to present findings on the nitric oxide scavenging (inflammatory) assay? IC50, percentage of inhibtion (%) OR nitrite equivalent (compared to sodium nitrite standard)?
Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...
11 December 2019 3,243 5 View
Hi, I am trying to culture a stable clones using transfected cells. As the clones grow, I noticed the eGFP signal diminishes over time but the cells are still resistant to antibiotics and do not...
11 December 2019 176 14 View
Hi all, I am new in lentivirus transduction work and have some problem as listed below: 1) Is CMV promoter considered a strong promoter to express the gene I want in the transgenic organism...
09 October 2019 8,592 4 View
Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...
08 September 2019 9,024 2 View
Hi Scientist, I am transfecting an oncogenic miRNA into a breast cancer cell line and based on your experience in the mammalian cell line, will such transfection affect the cell morphology?
06 July 2019 7,378 8 View
Hi, Can anyone recommend a plasmid extraction kit that could produce good plasmid yield (>300ng/uL). The plasmid I am using now is a high copy number plasmid and the bacteria I am using is...
06 July 2019 6,820 3 View
Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...
05 June 2019 5,879 7 View
Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.
05 June 2019 9,712 3 View
Hi all, I would like to seek for your opinions on whether fluorescence approach (GFP) or luciferase approach is better in assessing transfection efficiency / success (after miRNA...
03 April 2019 1,383 6 View
Hi researcher, I wonder any of you have tried to design a single plasmid that carries both miRNA and reporter gene like GFP / luciferase? I am thinking with such plasmid, I will just need to...
03 April 2019 5,144 7 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View
I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...
22 February 2021 5,324 3 View