Thanks Dr. Chinmaya Sadangi for the suggestion. I will try to ask if they are willing to provide any free extraction kits or not.

Dear Zx

There are quite a few options for this. What i would caution against would be multi-template extraction kits (e.g. those from Qiagen allowing you to extract DNA, RNA and Protein). Since brain tissue is fatty DNA and RNA extractions should go smoothly, however, the protein re-suspension will prove near impossible.

In terms of options it depends on budget: would you like to try salt-based extraction methods, phenol-chloroform, or a kit of sorts?

Regards,

Hi Nathaniel Wade Mcgregor , thanks for the comment.

I have Qiagen Blood DNA extraction kit in my lab and I am planning to use the same kit for the DNA extraction from brain tissue. My concern is that the protein/fat precipitates might not pass through the column filter smoothly, even after I have smashed and homogenized the tissue.

Do you have any recommendation that the brain tissue suspension (after homogenization) can pass through the column filter smoothly?

Thank you.

Dear Zx

If you are going to be extracting from whole-blood you will not be able to appropriately isolate proteins and fats in a three-way extraction protocal (even when using a kit). You will need to use brain tissue to get comparable protein template. That said, if you do use brain tissue and use the Qiagen Triple DNA/RNA/ Protein kit you will not get reliable results for the protein extractions since brain tissue is so fatty and compromises the output of comparable total protein during the extraction protocol.

You will therefore need to use separate extraction procedures for DNA & RNA, and then for total protein extraction. This is the only way to get reliable results [to the best of my knowledge at present anyway].

Regards,

Nathaniel Wade Mcgregor - we tried spooling DNA from frozen brain tissue samples that seems to be clean than kits. But now somethings they are very inconsistent in terms of results. Is there some way to remove lipids from genomic DNA?