How to quantify/calculate GC-MS analyzed data using isotope labelled (d3) standard?

Dear researchers I would like to learn how to quantify extract component A. I run extract at concentration of (0.2815 mg/ml). The extract was spiked with isotope labelled deuterated (A-d3)...

04 May 2019 5,839 1 View

Unwanted negative peaks in HPLC chromatogram ?

Dear fellows I am working on HPLC and run sample (mixture of 5 compounds) all were detected perfectly, but I am getting unwanted negative peaks at 2 mins after run? Can anyone figureout the issue?...

07 August 2018 8,656 1 View

Are there any diabetes marker cell lines?

I want to check the effect of different plant extracts using diabetic marker cell lines if anyone know kindly share information. Thanking in advance

03 April 2015 9,014 3 View

Anyone know about the best/optimum ratio of cells which is used for LPS-induced Nitric oxide production in RAW 264.7 cells using 24 well plate?

I am evaluating the anti-inflammatory effect of plant extracts and performed the experiment many times using RAW 264.7 cells but could not get the significant results. I want to know that how much...

02 March 2015 8,643 4 View

Can I use EtOAc:MeOH:Water in ratio of (100:20:1) for Sephadex LH-20? IF not then which solvent can I use as mobile phase for Sephadex LH-20 column?

I am isolating compounds from BuOH fraction and initially I did Silica gel column with mobile solvent was (EMW in 100:20:1) now I have plan to proceed with Sephadex LH-20 column. I want to know...

02 March 2015 183 4 View

First concentration (100 uL/ml) has lower average value while lower concentration (50 uL/ml) has higher average value? anyone know where error in exp?

11 December 2014 6,371 1 View

Does anyone know how to isolate compounds from Brassica oleracea group having n-Buoh fraction?

11 December 2014 9,484 6 View

Does anyone know how to check the activity of the plant extracts against Parkinson's disease for the in vitro models?

10 November 2014 7,460 3 View

Does anyone know an in-vitro protocol for inhibition of Monoamine Oxidase-A and B? can you send Protocol or share some useful articles?

10 November 2014 8,776 3 View

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10 November 2014 7,962 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View