How to quantify/calculate GC-MS analyzed data using isotope labelled (d3) standard?

Dear researchers I would like to learn how to quantify extract component A. I run extract at concentration of (0.2815 mg/ml). The extract was spiked with isotope labelled deuterated (A-d3)...

04 May 2019 5,839 1 View

Unwanted negative peaks in HPLC chromatogram ?

Dear fellows I am working on HPLC and run sample (mixture of 5 compounds) all were detected perfectly, but I am getting unwanted negative peaks at 2 mins after run? Can anyone figureout the issue?...

07 August 2018 8,656 1 View

Are there any diabetes marker cell lines?

I want to check the effect of different plant extracts using diabetic marker cell lines if anyone know kindly share information. Thanking in advance

03 April 2015 9,014 3 View

Anyone know about the best/optimum ratio of cells which is used for LPS-induced Nitric oxide production in RAW 264.7 cells using 24 well plate?

I am evaluating the anti-inflammatory effect of plant extracts and performed the experiment many times using RAW 264.7 cells but could not get the significant results. I want to know that how much...

02 March 2015 8,643 4 View

Can I use EtOAc:MeOH:Water in ratio of (100:20:1) for Sephadex LH-20? IF not then which solvent can I use as mobile phase for Sephadex LH-20 column?

I am isolating compounds from BuOH fraction and initially I did Silica gel column with mobile solvent was (EMW in 100:20:1) now I have plan to proceed with Sephadex LH-20 column. I want to know...

02 March 2015 183 4 View

First concentration (100 uL/ml) has lower average value while lower concentration (50 uL/ml) has higher average value? anyone know where error in exp?

11 December 2014 6,371 1 View

Does anyone know how to isolate compounds from Brassica oleracea group having n-Buoh fraction?

11 December 2014 9,484 6 View

Does anyone know how to check the activity of the plant extracts against Parkinson's disease for the in vitro models?

10 November 2014 7,460 3 View

Does anyone know an in-vitro protocol for inhibition of Monoamine Oxidase-A and B? can you send Protocol or share some useful articles?

10 November 2014 8,776 3 View

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10 November 2014 7,962 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View