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Questions related from Vikram Kumar
Suppose I want to prepare 0.1M sodium citrate buffer of pH 3.0, I observed that most people use citric acid monohydrate (as weak acid) and sodium citrate dihydrate (as sodium salt of weak acid)...
12 December 2023 8,228 3 View
I’ve queries regarding cryopreservation of mammalian cells in DMSO the queries are as fallows: Q) How DMSO will protect cells from intracellular ice formation during freezing? What I know is It...
09 June 2023 115 2 View
I've some queries regarding assigning passage no in cell culture In case of Adherent cells, If I fallow 1:2 split ratio then the no. of times passaged = no of times the cells are doubled ? For...
29 March 2023 5,678 6 View
I'd like to know that what are the different ways to know/identify whether a particular Gene is expressed or not ? Few points from my side are : 1) identifying it's corresponding m-RNA...
22 February 2023 6,242 4 View
I'd like to know What is the minimum and maximum amount of Plasmid DNA that can be used for transformation of Bacteria ?
20 February 2023 9,802 3 View
I'd like to know the procedure to recover DNA which was shipped on a filter paper as a spot ?
20 February 2023 8,472 3 View
Can we able to access editors/reviewers comments on a research paper ? If so How ? Also I’d like to know which journals publish the comments of the editor/reviewer in a scientific paper so that...
19 February 2023 8,020 4 View
Usually Antigen Binding site is located within variable region of Heavy chain and light chain, But in the antibody structure representation why the Heavy chain is on the top and Light chain is on...
03 December 2022 1,263 2 View
I'd like to know What are the Secondary metabolites that are produced by mammalian cells during their cultivation in a Bioreactor ?
03 December 2022 2,012 0 View
I'd like to know the Highest protein producing cell in the Body ? I guess It's B cells which produce antibodies, Any other comments ?
20 October 2022 5,346 5 View
Suppose you are trying to express a protein in Bacteria but that protein is coming in Inclusion bodies but not secreted out. So is it possible if I express that same protein in mammalian cell with...
20 October 2022 4,300 3 View
I've few queries regarding bacterial and mammalian plasmids for expression of Gene of Interest. What plasmid elements/components that are differ between bacterial and mammalian Plasmids to express...
20 October 2022 3,646 1 View
I'd like to know the Maximum Yield we can achieve with CHO cell line (Irrespective of CHO-s/ExpiCHO/CHO-K1 e.t.c and also the mode of operation like batch, Fed-batch and Perfusion) ?
19 October 2022 2,058 2 View
I want to purify Peptide-Fc Conjugate through Affinity purification, So I tried using Mab Select Sure which is a Protein A column used for purification of mAb's, But during elution there was no...
16 October 2022 8,512 4 View
I'd like to know how to track/follow the flow path through the sample pump, to the column and into our fraction collector in case of AKTA Avant? I mean what are the different methods/Techniques...
07 September 2022 8,897 0 View
In research papers people plot the trend of different parameters( Temp, pH, %DO, Air Flow rate) of Bioreactor, what all points we should focus. Few points from my side are : The X- and Y- axis...
29 July 2022 5,549 0 View
I tried to express a protein of interest in mammalian cells and after it's extracellular secretion in the supernatant and purification I'ld like to know whether it is properly/correctly folded or...
27 June 2022 5,724 4 View
I've few queries about Transfection of ExpiCHO Cells 1) Is there any Specific plasmid vector (that contains gene of interest) that was suitable to transfect ExpiCHO cells ? 2) Can I transfect...
22 June 2022 5,414 0 View
I'ld like to know the list of all the Research papers that uses ExpiCHO Expression system /ExpiFectamine™ CHO Transfection Kit (from Thermo Scientific) ?
22 June 2022 2,622 0 View
I'd like to know the different type of post translational modifications that occur in a mammalian cell ? Few examples from my side are : Glycosylation. Phosphorylation. Ubiquitination Epigenetic...
18 April 2022 2,434 4 View
What are the different ways to Balance odd number of tubes within 12 positions of a centrifuge ?
10 March 2022 4,548 1 View
I'ld like to know how much similarity a molecule should show with innovator molecule in order to consider it as a Biosimilar ?
26 January 2022 2,866 4 View
I'ld like to know what is the difference between ExpiCHO and CHO-S cell line ?
22 January 2022 3,926 3 View
I'ld like to know why 293 number is mentioned along with HEK, why it is called HEK 293, what does 293 will represent/indicate ?
22 January 2022 408 3 View
I'ld like to know what is the mechanism of action of Anticlumping agent that prevents cells from forming clumps ?
22 January 2022 9,747 2 View
I performed restriction double digestion of my plasmid with ECORV-HF and PacI with the fallowing reaction protocol : (Source : NEB Cloner) Steps Set up reaction as follows: COMPONENT : Total 50...
27 December 2021 7,175 5 View
Suppose I've to prepare a 100 mL of 70% ethanol by mixing 70 mL of ethanol and 30 mL of water, I'ld like to know is there any difference between 1st pouring large volume (70 mL) next small volume...
28 October 2021 174 3 View
It was known that mixing equal volume say for example 25 mL of ethanol and 25 mL water doesnot result in 50 mL final volume of the final mixture. Suppose I'ld like to prepare 100 mL of 70%...
28 October 2021 8,182 3 View
I'm cultivating suspension mammalian cells in a bioreactor, I'ld like to know what all the methods to detect/know whether it is contaminated or not. Few points from my side are : 1) DO (Disolved...
28 October 2021 3,124 6 View
I overexpressed a particular protein in CHO cells and I'ld like to know how to detect/know/confirm that secretion is the bottleneck for it's low yiled/production ? One point from my side...
24 October 2021 1,366 3 View
I'ld like to know what are the different Ways to reduce operational cost during a monoclonal antibody production process development ?
26 September 2021 1,078 1 View
Suppose I've given u two samples in which one sample contains live cell and other sample contains dead mammalian cells (Example CHO), Since both the samples are not labelled, I'ld like to know...
04 September 2021 3,096 4 View
I transfected the CHO (Chinese hamster ovary) Cells with Plasmid containg Heavy and light chai genes of a mAb, I'ld like to know during which phase of cell growth the mAb will be produced ?
01 September 2021 5,552 2 View
Generally CHO (DHFR -ve) cells on transfection with plasmid bearing DHFR gene + Gene of Interest and upon addition of MTX only thoose cells which take up plasmid (containing DHFR + Gene of...
22 July 2021 7,686 3 View
1) In the Freedom CHO-S kit user guide it was mentioned that CHO-S cells contain basal expression of DHFR, upon transfection how to distinguish b/n untransfected and transfected cells which take...
22 July 2021 5,927 0 View
I'm culturing Chinese hamster ovary (CHO) cells in CD. FortiCHO™ Medium + Glutamax, I'm fallowing "Freedom™ CHO-S™ Kit USER GUIDE For transfection of CHO-S™ Cells (cGMP-banked) and development...
14 July 2021 3,357 0 View
I'ld like to know what all the difference between a Folded and Un Folded protein ? Few points from my side are : Folded Proteins are : Biologically Active, 3D Structure, Hydrophobic residues...
13 June 2021 1,535 5 View
Suppose I've given you an adherent cell line and a suspension cell line and asked to perform the MTT Assay of Both the Cells together, Then how will you Perform ?
01 June 2021 2,055 2 View
I would like to know what are different possible ways of acquiring antibodies apart from exposure to foreign Antigen. Few examples are : Acquiring antibodies from 1)Mother through placenta and...
29 March 2021 7,722 3 View
I want to develop stable cell line for protein production, for that i linearized the plasmid containing Gene of Interest and Antibiotic Resistance gene for Geneticin and Transfected into CHO cells...
28 March 2021 1,536 3 View
Generally we produce protein (which requires Folding and Glycosylation) in large scale by expressing the protein in Mammalian cells (mostly CHO cells), Suppose if the protein expressed and...
28 March 2021 1,043 3 View
