Suppose I've given you an adherent cell line and a suspension cell line and asked to perform the MTT Assay of Both the Cells together, Then how will you Perform ?
In addition, such an experiment would be technically impossible to do. The MTT test requires the formation of crystals of pharmacan from MTT and their subsequent solubilization (DMSO, isopropanol). The suspension cells themselves in the wells of the plate will distort the optical density, which is measured by the plate reader as a result of dissolution of the crystals of pharmacan.