I transfected the CHO (Chinese hamster ovary) Cells with Plasmid containg Heavy and light chai genes of a mAb, I'ld like to know during which phase of cell growth the mAb will be produced ?
It may depend on your vector, but usually CHO cell expression would be constitutive. That means, you want to give them as much time as possible to grow and accumulate antibody in the culture medium. Until the medium is totally depleted and the cells are just about to start dying.
At the transition from lag to log phase during which at this point the cells have enough energy to synthesize DNA and other proteins for themselves which would also include the protein of interest as in this case the mAb.