I've few queries regarding bacterial and mammalian plasmids for expression of Gene of Interest. What plasmid elements/components that are differ between bacterial and mammalian Plasmids to express a gene of Interest.
According to me :
The elements/components that are common between bacterial and mammalian Plasmids are :
The elements/components that are differ between bacterial and mammalian Plasmids are:
I'd like to know is there any other differences?
Thank You.
Plasmids for mammalian expression use different organism specific promoters, a eukaryotic ribosomal binding site, an intron in the CDS of the gene of interest to avoid bacterial expression and to increase expression in the mammalian cells and a poly A tail after the stop codon to reduce mRNA degradation. They also usually have a second selective marker cassette so they can be selected in both bacteria and in the mammalian cell line.
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Materials Science
- Materials
- Inorganic Materials
- Metals
- Alloys
- Steel