Any colorimetric phagocytosis assays?

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XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints. in K alpha2 value..can anyone help?

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints when entering K alpha value. Because of that I am not able to find the peaks...

11 June 2024 4,018 2 View

Crystal violet or hematoxylin is preferred for the staining of the Transwell membrane?

Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?

04 June 2024 2,766 0 View

Will a longer incubation of THP1-derived M2 macrophages on the assay plate have an impact on phagocytosis?

I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...

10 January 2024 538 1 View

How do we plot gain versus frequency plot in CST tool while designing an antenna?

I think there is no direct option to plot gain versus frequency in CST. kindly give solution

09 October 2023 8,738 3 View

What is the effect of DMSO in calcium assays?

What is the impact of DMSO on calcium influx, and why does it lead to a significant decrease in fluorescence when compared to the control group without treatment during ATP-induced Fluo-4 assay...

28 September 2023 7,446 2 View

What is the optimized protocol for the Fluo 4 NW calcium assay in THP1-differentiated macrophages?

I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks

13 July 2023 4,985 0 View

What are the ideal mouse prostate cancer cell line to use in C57BL/6 mice?

I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.

13 June 2023 7,991 1 View

Any protocol or kit to measure intracellular calcium levels using a fluorescence microplate reader?

I am planning to measure the intracellular calcium level in THP1-derived macrophages and am looking for a protocol or kit to do so.

26 April 2023 2,036 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View