Hi

PCR condition are fully explained in our paper

http://www.ncbi.nlm.nih.gov/pubmed/15161705

with what material did you try to optimize your PCR ? cDNA ?

you should perhaps begin to check the primer with a plasmid that contain the TP53 cDNA for optimization

Best

T

Hi Thierry,

Thank you so much. yes, I tried with human cDNA.

I will check your paper and also will try with p53 positive plasmid.

Thanks again

Hi

can't we optimize it with Taq polymerase  instead of PFU?