I have the M1 and M2a subtypes of macrophages that I differentiated and polarized from THP-1 monocytes. I wanted to know the most suitable phagocytosis assays (preferably colorimetric method), migration assays, and proliferation assays.
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...
16 July 2024 8,862 2 View
In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints when entering K alpha value. Because of that I am not able to find the peaks...
11 June 2024 4,018 2 View
Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?
04 June 2024 2,766 0 View
I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...
10 January 2024 538 1 View
I think there is no direct option to plot gain versus frequency in CST. kindly give solution
09 October 2023 8,738 3 View
What is the impact of DMSO on calcium influx, and why does it lead to a significant decrease in fluorescence when compared to the control group without treatment during ATP-induced Fluo-4 assay...
28 September 2023 7,446 2 View
I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks
13 July 2023 4,985 0 View
I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.
13 June 2023 7,991 1 View
I am planning to measure the intracellular calcium level in THP1-derived macrophages and am looking for a protocol or kit to do so.
26 April 2023 2,036 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...
01 August 2024 476 2 View
I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...
29 July 2024 236 2 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?
18 July 2024 5,214 4 View