Need for PDF opportunity in good University?

I just completed my Ph.D. very recently. I am looking for any PDF opportunity at a foreign university. I have some decent publication in the field of solar pv systems. Can anyone suggest me some...

16 April 2019 7,007 1 View

Applying heat transfer analysis in turbine blade what are the boundary conditions we can use?

I'm now doing my final project about thermal barrier coating in turbine blade. For the optimization of TBC in turbine blade I'm going to make heat transfer analysis.Now I want the boundary...

01 March 2019 2,812 3 View

Can we do RT PCR with basal p53 expression?

I have extracted RNA from tumor tissue sample and wondering whether the basal level of p53 expression enough to do RT PCR amplification using p53 transcript specific primers? Thank you

20 September 2018 3,669 3 View

Can anyone suggest model antigen-antibody pairs (best studied models for direct and sandwich immunoassay)?

We have developed a fiber optic sensor and we wish to evaluate them for biosensing. Is there any standard antigen-antibody pair for immunoassay, namely direct and sandwich formats. For example...

18 December 2017 6,541 3 View

Suggest any suitable technique for making 6mm dia. hole on ~4mm thickness fiber composite without any delamination.

19 June 2017 928 7 View

What is the abbreviation of TECS (It is a hard fluoropolymer used as clading in silica optical fibers)?

05 April 2017 4,495 2 View

Can anyone suggest better protocol for extraction of yeast RNA?

03 October 2016 8,147 3 View

Microfiltration for enzyme liquid?

I want to achieve a microclear (free of any bacteria & fungi) enzyme liquid, below are parameters of my feed Feed Sample: Papain Enzyme liquid pH: 4.5 to 5.8 Total Suspended Solids: 9% to...

22 July 2016 6,546 1 View

P53 mutation status of MX-1 breast cancer cell line?

Is MX-1 breast cancer cell line P53 wild type or mutant?

28 January 2016 2,433 3 View

Which will be the most suitable medium to culture MX-1 breast cancer cell line?

24 November 2015 8,377 2 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View