What genes are good qPCR markers for macrophage differentiation?

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XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Convergence criteria not met but ends with normal termination. Could not open the log file in gaussview. How to rectify this problem?

>>>>>>>>>> Convergence criterion not met. SCF Done: E(RB3LYP) = -472.437545326 A.U. after 33 cycles NFock= 32 Conv=0.29D-07 -V/T= 2.2914...

16 July 2024 8,862 2 View

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints. in K alpha2 value..can anyone help?

In XRD analysis using Xpert highscore plus software I am getting error as Index < 0 or Index >= NrOfDataPoints when entering K alpha value. Because of that I am not able to find the peaks...

11 June 2024 4,018 2 View

Crystal violet or hematoxylin is preferred for the staining of the Transwell membrane?

Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?

04 June 2024 2,766 0 View

Will a longer incubation of THP1-derived M2 macrophages on the assay plate have an impact on phagocytosis?

I am planning to perform a phagocytosis assay for THP1-derived M2 macrophages. According to the Vybrant phagocytosis assay kit, it says to incubate the cells in the assay plate for an hour before...

10 January 2024 538 1 View

How do we plot gain versus frequency plot in CST tool while designing an antenna?

I think there is no direct option to plot gain versus frequency in CST. kindly give solution

09 October 2023 8,738 3 View

What is the effect of DMSO in calcium assays?

What is the impact of DMSO on calcium influx, and why does it lead to a significant decrease in fluorescence when compared to the control group without treatment during ATP-induced Fluo-4 assay...

28 September 2023 7,446 2 View

What is the optimized protocol for the Fluo 4 NW calcium assay in THP1-differentiated macrophages?

I am planning to measure ca influx of an ion-gated receptor treated with its agonist and antagonist in THP1 differentiated macrophages. and looking for an optimized protocol. Thanks

13 July 2023 4,985 0 View

What are the ideal mouse prostate cancer cell line to use in C57BL/6 mice?

I am currently using the TRAMPC2 cells, but I am looking for any other cell lines I can use in C57BL/6 mice.

13 June 2023 7,991 1 View

Any protocol or kit to measure intracellular calcium levels using a fluorescence microplate reader?

I am planning to measure the intracellular calcium level in THP1-derived macrophages and am looking for a protocol or kit to do so.

26 April 2023 2,036 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Human macrophages cell surface markers?

I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...

29 July 2024 236 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

Staining of foam cells derived from PBMC differentiated to macrophages. Does anyone have experience with this type of assay?

Hi everyone, I am trying to induce foam cells in macrophage differentiated from PBMC. Which type of staining do you prefer to use? Nile Red or Oil RED O ? What concentration and timing of ox-LDL...

01 July 2024 8,286 0 View

Is there any in-vitro method to test the Immunogenicity of a novel Antigen?

I am trying to quantify the release of Nitric oxide to test the Immunogenicity of my antigen on U937 cells (Monocytes). Based on the literature, I am supposed to convert 1x105 cells/ml to...

27 June 2024 3,131 2 View

How often should I change media for RAW 264.7 cell cytokine analysis?

I am currently working on a study using RAW 264.7 cells. I am polarizing the cells to an M1 phenotype. I am then applying an anti-inflammatory compound 24 hours later. These cultures are being...

09 June 2024 867 1 View

Fereshteh Nazari Khanamiri

I hope this can be helpful for you:

https://www.sciencellonline.com/genequerytm-human-macrophage-polarization-markers-qpcr-array-kit.html

Alexander Trampe

This publication by Murray et al. (Immunity. 2014 Jul 17;41(1):14-20.) may also be useful to you:

Article Macrophage Activation and Polarization: Nomenclature and Exp...