Need for PDF opportunity in good University?

I just completed my Ph.D. very recently. I am looking for any PDF opportunity at a foreign university. I have some decent publication in the field of solar pv systems. Can anyone suggest me some...

16 April 2019 7,007 1 View

Applying heat transfer analysis in turbine blade what are the boundary conditions we can use?

I'm now doing my final project about thermal barrier coating in turbine blade. For the optimization of TBC in turbine blade I'm going to make heat transfer analysis.Now I want the boundary...

01 March 2019 2,812 3 View

Can we do RT PCR with basal p53 expression?

I have extracted RNA from tumor tissue sample and wondering whether the basal level of p53 expression enough to do RT PCR amplification using p53 transcript specific primers? Thank you

20 September 2018 3,669 3 View

Can anyone suggest model antigen-antibody pairs (best studied models for direct and sandwich immunoassay)?

We have developed a fiber optic sensor and we wish to evaluate them for biosensing. Is there any standard antigen-antibody pair for immunoassay, namely direct and sandwich formats. For example...

18 December 2017 6,541 3 View

Suggest any suitable technique for making 6mm dia. hole on ~4mm thickness fiber composite without any delamination.

19 June 2017 928 7 View

What is the abbreviation of TECS (It is a hard fluoropolymer used as clading in silica optical fibers)?

05 April 2017 4,495 2 View

Can anyone suggest better protocol for extraction of yeast RNA?

03 October 2016 8,147 3 View

Microfiltration for enzyme liquid?

I want to achieve a microclear (free of any bacteria & fungi) enzyme liquid, below are parameters of my feed Feed Sample: Papain Enzyme liquid pH: 4.5 to 5.8 Total Suspended Solids: 9% to...

22 July 2016 6,546 1 View

P53 mutation status of MX-1 breast cancer cell line?

Is MX-1 breast cancer cell line P53 wild type or mutant?

28 January 2016 2,433 3 View

Which will be the most suitable medium to culture MX-1 breast cancer cell line?

24 November 2015 8,377 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Can anyone help me with doing resorption pit assay on biomaterials for 28 days?

I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...

01 February 2021 6,746 2 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

What are the validation parameters for RAW BLUE macrophages 264.7?

Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...

24 January 2021 589 2 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View

No title

Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

Has anybody had staining problems with lysotracker in live cells?

I am working with two fish cells types, a zebrafish liver cell line and primary cultures of trout macrophages. We are using lysotracker to stain them in order to visualize lysosomes in confocal...

16 December 2020 3,935 2 View

My Wright-Giemsa Stain on macrophages isn't showing any cytoplasm, just nucleus. Any tips to improve my staining?

I'm pretty sure they are mostly macrophages since after a 2 hour incubation in M199 they are stuck with pseudopodia. My slides just aren't pretty enough for me. I'm using Fisherbrand Wight-Giemsa...

13 December 2020 5,475 3 View