How long after Critical Point Drying (CPD) step, samples still good for SEM?

Anyone has experience about this? Thank in advance.

09 October 2016 2,625 0 View

Fix OsO4 for SEM preparation sample?

06 July 2016 8,658 4 View

Measurement accurate amount of components in rice seed (starch, protein and lipid)?

Do anyone can suggest me the effective method to analysis components in rice seed, especially starch? Thank you in advance.

06 July 2016 6,615 6 View

Is anyone familiar with Indica and Japonica flag leaf?

03 April 2015 6,889 2 View

How do I determine the transcription start site?

I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...

02 March 2015 6,893 7 View

Can anybody help me to design a primer for cloning?

09 October 2014 8,727 5 View

Does anyone have experience with sampling pollen?

07 August 2014 3,560 2 View

Can anyone tell the protocol for stomatal movement in rice?

Please support more details

06 July 2014 2,755 0 View

Can anyone help with concentration of jasmonic acid/methyl jasmonate in vitro?

For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?

04 May 2014 9,337 1 View

How can I detect a phenotype?

I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...

03 April 2014 309 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View