I want to clone around 4kb fragment, can I make it with T-easy vector (3kb) or do you have another suggestion?

12 December 2013 1 10K Report

In principle it should be possible, though you may suffer a bit on the efficiency side as you go up on the size of the insert, and 4kb is a fair size. The Promega PGEM T-easy however is a good cloning system. Of course your insert has to be a PCR product.

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