I used Ubiquitin 5 as control primer to check balance cDNA and I got a good result. But when I tried to check with another control primer like Ubiquitin 1 or OsActin, those bands were different (if among 3 control primers, we can get 2 in the same result, and then go to next step). What should I do now? Should I try to find better control primers or should I try to get balance from Ubiquitin 1 result?
Hi Tuyet..
I also agree with Yang...go for some genes which are known as stable reference genes in rice...I have come across a pape where the research group has compared few housekeeping genes in rice to see their utility as reference genes in Rice and Wheat..I will send you the paper if I get hold of it..Since all house keeping gene may not be stable always in all conditions...so all may not be good candidates for a reference gene....some as you have also seen may affect the calculations....rice is a model plant and a lot of work has been done..so choose a well studied gene.....eLF-1alpha and GAPDH may also serve as good controls...
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