31 Questions 146 Answers 0 Followers
Questions related from Thomson Patrick Joseph
Recently, through social media i came across several issues of article retraction. Where, blots were even duplicated, FACS results were duplicated, blots were manipulated and so on.... What are...
03 March 2018 7,807 4 View
Hello, Which software can be used (free) for the analysis of micro-array data other than GESA and GenePattern ? Best Regards
02 February 2018 4,176 3 View
What is the difference between HEK293 and HEK293T cells? HEK293T is also written as 293T cells? Thanks Regards
12 December 2017 3,377 1 View
For the whole mitochondrial genome sequencing, how i can achieve the good quality of mt DNA from animal tissue? Any suggestion Regards
12 December 2017 2,661 2 View
Hello, What method can be used for the determination of complex I activity of mitochondria (cultured cells/fresh tissue)? Thanks Best Regards
12 December 2017 7,821 4 View
Hello, How a beginner researcher can find the opportunity of writing a chapter for book in his/her field of interest? Thanks Best Regards
11 November 2017 7,358 2 View
If the papers are published before writing thesis, and in that case if the material from papers are copied into thesis are considered as self plagiarism? Regards
11 November 2017 8,451 7 View
Hello, What is advisable for a PhD student to write thesis first or publish first? or both can be done simultaneously? Best Regards
11 November 2017 9,423 4 View
Which software can be used for PCA analysis? where i can draw the graph and interpretate it? Any guideline how PCA analysis can be done using SPSS... Regards
11 November 2017 6,601 20 View
HepG2 cells are Hepatocellular Carcinoma (HCC) cells or hepatoblastoma cells? Is this cell line really a misidentified cell line? Regards
11 November 2017 6,213 4 View
HEK-293T cell line can be used as normal cell line if i am investigating the anticancer potential of a compound against a panel of cancerous cell line (considering HEK-293T as normal among the...
11 November 2017 4,517 3 View
What is the shortest, easiest and the effective way of making cell line resistant to a particular drug/inhibitor? Regards
11 November 2017 1,156 3 View
What is the criteria of IF distribution between the authors? It varies from institution to institution or there is any fix rule? If the IF of journal is 5.0 how it will be distributed between...
11 November 2017 2,600 4 View
What are the different methods of in vitro and in vivo drug delivery? Which method should be preferred if the drug is recombinant protein? Best Regards
11 November 2017 8,231 5 View
How is writing thesis different from writing a paper? Please share your experience and opinion ..... Regards
11 November 2017 8,229 12 View
What is the basic difference between between the SCI (Science Citation Index) Journal and SCIE (Science Citation Index Expanded)? Does it matters in case of quality of journal? Thanks Regards
11 November 2017 1,628 9 View
Just don’t call your results “significant.” Call them “suggestive” instead. The change of the value of significance from < 0.05 to < 0.005 will improve the reproducibility of scientific...
11 November 2017 1,896 5 View
GAPDH can used as loading control for nuclear fraction in western blotting? Regards
10 October 2017 3,329 2 View
What are the conditions in which wet transfer is preferred over dry or semi dry transfer in western blotting? Regards
10 October 2017 5,157 4 View
Which one to go for CRISPR or RNAi, for knocking down a single gene in cancer cell line? Thanks in advance.....
10 October 2017 3,594 1 View
Solubilization of inclusion body can be done between 20-30 degree with mild solublization buffer? What could be the best time/temperature combination for achieving maximum solubilization? Best Regards
10 October 2017 1,397 2 View
Please suggest any free and reliable online software for protein nucleic acid docking (for predicting the coupling between protein and nucleic acid? Regards
10 October 2017 8,077 3 View
Any guideline for the conversion of PhD thesis into book?Please also mention the details of publisher that can help in this regard. Many Thanks
09 September 2017 7,490 4 View
Any web server for the prediction of active site of recombinant protein (enzyme) using amino acid sequence? Regards
08 August 2017 1,894 4 View
Where mutation status of cancer cell lines can be checked? other then CCLE.
01 January 1970 2,218 2 View
For the linearization of pPic9 plasmid i am using SacI enzyme, but its coming in AoxI promoter, it if effect the recombination and expression of my protein? or i can go with it Regards
01 January 1970 6,738 2 View
I am using E-coli as expression system, and i am getting 12-16 mg of protein from 1 liter of culture, is it reasonable? In percentage the overall yield is coming out to be around 25.87%.
01 January 1970 3,386 0 View
I am digesting pET32a+ with EcoRI and XhoI (TAKARA) a 37 C for 1h in 50 ul reaction volume. In uncut plasmid i am getting three band however after digestion i got one band only ...no band for...
01 January 1970 7,478 3 View
I determined the Tm profile of my protein of interest using ABI qPCR machine under different buffer, salt conditions. Can i merge the file generated by ABI machine on expression suite software in...
01 January 1970 6,185 0 View
How people get error bar in control group of cell viability assay (CCK-8)? even if the experiment is run in triplicate, control will remain 100%...right?
01 January 1970 10,142 4 View
I am working on a recombinant protein that is having anticancer activity. Bioinformatic analysis, expression and little bit of activity part i have submitted in one journal, and planing to submit...
01 January 1970 1,453 5 View
