Loading control for nuclear fraction?

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Reason (s) for retraction of articles?

Recently, through social media i came across several issues of article retraction. Where, blots were even duplicated, FACS results were duplicated, blots were manipulated and so on.... What are...

02 March 2018 7,843 4 View

Microarray Data Analysis?

Hello, Which software can be used (free) for the analysis of micro-array data other than GESA and GenePattern ? Best Regards

01 February 2018 4,201 3 View

HEK293 and HEK293T cell lines?

What is the difference between HEK293 and HEK293T cells? HEK293T is also written as 293T cells? Thanks Regards

11 December 2017 3,429 1 View

Complex I activity determination of mitochondria?

Hello, What method can be used for the determination of complex I activity of mitochondria (cultured cells/fresh tissue)? Thanks Best Regards

11 December 2017 7,862 4 View

Mitochondrial DNA and whole mitochondrial genome sequencing?

For the whole mitochondrial genome sequencing, how i can achieve the good quality of mt DNA from animal tissue? Any suggestion Regards

11 December 2017 2,695 2 View

Principle Component Analysis (PCA) Analysis?

Which software can be used for PCA analysis? where i can draw the graph and interpretate it? Any guideline how PCA analysis can be done using SPSS... Regards

10 November 2017 6,645 20 View

Difference between the SCI (Science Citation Index) Journal and SCIE (Science Citation Index Expanded)?

What is the basic difference between between the SCI (Science Citation Index) Journal and SCIE (Science Citation Index Expanded)? Does it matters in case of quality of journal? Thanks Regards

10 November 2017 1,681 9 View

Criteria for the distribution of Impact Factor between author(s)?

What is the criteria of IF distribution between the authors? It varies from institution to institution or there is any fix rule? If the IF of journal is 5.0 how it will be distributed between...

10 November 2017 2,638 4 View

Paper should be written before/after thesis?

Hello, What is advisable for a PhD student to write thesis first or publish first? or both can be done simultaneously? Best Regards

10 November 2017 9,454 4 View

HEK-293T can be used as normal cell line?

HEK-293T cell line can be used as normal cell line if i am investigating the anticancer potential of a compound against a panel of cancerous cell line (considering HEK-293T as normal among the...

10 November 2017 4,543 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ?

Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...

25 July 2024 7,520 0 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

How Can I Resolve a Persistent Unwanted NMR Peak at 1.25 ppm?

Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...

15 July 2024 9,065 4 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Is there anyway to distinguish two proteins of same molecular weight?

I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...

14 July 2024 9,324 2 View

What would you do next?

Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...

12 July 2024 6,313 0 View