I am using E-coli as expression system, and i am getting 12-16 mg of protein from 1 liter of culture, is it reasonable? In percentage the overall yield is coming out to be around 25.87%.
Recently, through social media i came across several issues of article retraction. Where, blots were even duplicated, FACS results were duplicated, blots were manipulated and so on.... What are...
02 March 2018 7,843 4 View
Hello, Which software can be used (free) for the analysis of micro-array data other than GESA and GenePattern ? Best Regards
01 February 2018 4,201 3 View
What is the difference between HEK293 and HEK293T cells? HEK293T is also written as 293T cells? Thanks Regards
11 December 2017 3,429 1 View
Hello, What method can be used for the determination of complex I activity of mitochondria (cultured cells/fresh tissue)? Thanks Best Regards
11 December 2017 7,862 4 View
For the whole mitochondrial genome sequencing, how i can achieve the good quality of mt DNA from animal tissue? Any suggestion Regards
11 December 2017 2,695 2 View
Which software can be used for PCA analysis? where i can draw the graph and interpretate it? Any guideline how PCA analysis can be done using SPSS... Regards
10 November 2017 6,645 20 View
What is the basic difference between between the SCI (Science Citation Index) Journal and SCIE (Science Citation Index Expanded)? Does it matters in case of quality of journal? Thanks Regards
10 November 2017 1,681 9 View
What is the criteria of IF distribution between the authors? It varies from institution to institution or there is any fix rule? If the IF of journal is 5.0 how it will be distributed between...
10 November 2017 2,638 4 View
Hello, What is advisable for a PhD student to write thesis first or publish first? or both can be done simultaneously? Best Regards
10 November 2017 9,454 4 View
HEK-293T cell line can be used as normal cell line if i am investigating the anticancer potential of a compound against a panel of cancerous cell line (considering HEK-293T as normal among the...
10 November 2017 4,543 3 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am currently writing a systematic review
15 July 2024 9,176 1 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View
I studying PhD course in Urban design for Health. I need to know in some fileds: residental block, green open space and Transport TOD. I need connect with expert in that fileds.
14 July 2024 1,892 3 View