I am using E-coli as expression system, and i am getting 12-16 mg of protein from 1 liter of culture, is it reasonable? In percentage the overall yield is coming out to be around 25.87%.
Recently, through social media i came across several issues of article retraction. Where, blots were even duplicated, FACS results were duplicated, blots were manipulated and so on.... What are...
02 March 2018 7,715 4 View
Hello, Which software can be used (free) for the analysis of micro-array data other than GESA and GenePattern ? Best Regards
01 February 2018 4,073 3 View
What is the difference between HEK293 and HEK293T cells? HEK293T is also written as 293T cells? Thanks Regards
11 December 2017 3,268 1 View
Hello, What method can be used for the determination of complex I activity of mitochondria (cultured cells/fresh tissue)? Thanks Best Regards
11 December 2017 7,703 4 View
For the whole mitochondrial genome sequencing, how i can achieve the good quality of mt DNA from animal tissue? Any suggestion Regards
11 December 2017 2,564 2 View
Which software can be used for PCA analysis? where i can draw the graph and interpretate it? Any guideline how PCA analysis can be done using SPSS... Regards
10 November 2017 6,501 20 View
What is the basic difference between between the SCI (Science Citation Index) Journal and SCIE (Science Citation Index Expanded)? Does it matters in case of quality of journal? Thanks Regards
10 November 2017 1,460 9 View
What is the criteria of IF distribution between the authors? It varies from institution to institution or there is any fix rule? If the IF of journal is 5.0 how it will be distributed between...
10 November 2017 2,500 4 View
Hello, What is advisable for a PhD student to write thesis first or publish first? or both can be done simultaneously? Best Regards
10 November 2017 9,329 4 View
HEK-293T cell line can be used as normal cell line if i am investigating the anticancer potential of a compound against a panel of cancerous cell line (considering HEK-293T as normal among the...
10 November 2017 4,416 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...
01 March 2021 6,187 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View
Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?
28 February 2021 3,653 4 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...
27 February 2021 947 7 View
Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?
26 February 2021 3,487 3 View
I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.
21 February 2021 6,523 6 View
What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...
21 February 2021 1,831 4 View