How people get error bar in control group of cell viability assay (CCK-8)? even if the experiment is run in triplicate, control will remain 100%...right?
they are two ways of presenting data, first like what have shown, presenting the raw data as individual graph/bar. In that way, every data from each sample will have its own mean and error. The second way of presenting is by normalising first against the negative control. In this manner, we do not see any graph/bar representing negative control because each of the data obtained from each samples is divided against the negative control which will give the output as percentage comparison with negative control.
yes i agree with statement of yours, but here they are presenting the percent viability not the absorbance they got for control and test groups. say for example if the mean abs of control is 0.456, it will be multiplied with 100 and divided by 0.456 so as to get %age....right? and it will come out to be 100%....and same thing happens for experiments run in triplicate....my question/confusion is about error bar in control group...because if % viability in control for three experiments is 100 then how one can get error bar...
please correct me if i am understanding wrong...
regards
I agree with you. I did not think of it when answering this.
Then I think it is possible that they get the mean of the control group as the denominator to normalise each and every value that they get, including the control group triplicate values. I wonder whether that is the right thing to do.
yes i thought the same, but if they do so for the control group they should follow the same for test as well.....Many thanks Win Sen Heng...
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