25 Questions 79 Answers 0 Followers
Questions related from Thomson Patrick Joseph
Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...
02 May 2024 3,108 0 View
Hi I am investing the calcium signaling using Fluoro 4 in human iPSC derived motor neurons. While living imaging cells, how could I set the confocal parameters? so as to know at what calcium...
08 September 2023 4,382 1 View
What could be the best controls in immunofluorescence? The tissue i am using is zebrafish brain. Thanks Best Regards
16 October 2019 8,172 1 View
Two morpholinos targeting paralogs of a gene in zebrafish can be micro injected at the same time (mix together and injected)? Thanks Best Regards
22 February 2019 4,222 4 View
Cell lines after going through number of passage trends to change. Is there any fixed duration after which cell lines should be authenticated (by STR profiling etc)?How often one should...
17 January 2018 7,975 2 View
03 January 2018 1,284 3 View
HL-60 cell line should be referred as Adult acute myeloid leukemia or Human promyelocytic leukemia? In the below mentioned link it is referred as Adult acute myeloid leukemia cells however Human...
30 December 2017 7,754 2 View
For the whole mitochondrial genome sequencing, how i can achieve the good quality of mt DNA from animal tissue? Any suggestion Regards
22 December 2017 9,710 1 View
HepG2 cells are Hepatocellular Carcinoma (HCC) cells or hepatoblastoma cells? Is this cell line really a misidentified cell line? Regards
28 November 2017 6,466 3 View
HEK-293T cell line can be used as normal cell line if i am investigating the anticancer potential of a compound against a panel of cancerous cell line (considering HEK-293T as normal among the...
27 November 2017 9,917 3 View
Hello, where one can predict the interaction between glycan and glycan binding protein (GBP)? Any database or free software ? Thanks
06 November 2017 9,927 2 View
Which protocol can be used for the isolation of circulating tumor cells from patients? Best Regards
05 November 2017 5,928 4 View
What are the growth factor that can be added in the culture media in order to support the growth of Circulating tumor cells? Regards
05 November 2017 7,866 2 View
Anybody have experience of using calcium phosphate method for HEK-293T transfection? How about the efficiency and the long term effects on cells? Regards
30 October 2017 2,533 2 View
What criteria should be used for the validation of homology modeling? Considering z-score, RMSD value and Ramachandan plot analysis is enough/reliable for validation of homology model? Best Regards
30 October 2017 1,745 10 View
Flexible docking can be done by using MOE? Please share your experience if flexible docking (i.e. flexible side chains) is possible on MOE...... Best Regards
30 October 2017 4,646 6 View
What is the minimum and maximum nucleotide length between two loxP sites in Cre recombinase system? Best Regards
07 October 2017 4,039 2 View
Up to what extent and how his tag effect the expression, purification and structure of a recombinant protein? Regards
02 October 2017 2,172 8 View
How i can check/detect the off target effects while using CRISPR Cas9?
02 October 2017 8,768 5 View
Hello Can prolonged gene silencing effect be achieved by repeated siRNA transfecions?
28 September 2017 5,469 4 View
Net-NGlyc 1.0 server, Net-OGlyc 3.1 server, NetPhos 3.1 server can be used of the analysis of mushroom originated proteins? If yes, how one can justify? If no whats the reason behind it Regards
26 September 2017 7,338 2 View
Which database/web server can be used for the prediction of protein function originated from mushroom ? Regards
26 September 2017 9,660 2 View
Dormancy of the cancer cells results in the recurrence of disease, after treatment of the primary tumor. What might be the possible factors responsible for this phenomena and how these property of...
22 September 2017 7,155 4 View
For the linearization of pPic9 plasmid i am using SacI enzyme, but its coming in AoxI promoter, it if effect the recombination and expression of my protein? or i can go with it Regards
04 September 2017 2,714 2 View
I am working on a recombinant protein that is having anticancer activity. Bioinformatic analysis, expression and little bit of activity part i have submitted in one journal, and planing to submit...
21 August 2017 1,845 5 View
