Controls for immunofluorescence?

16 October 2019 1 8K Report

What could be the best controls in immunofluorescence? The tissue i am using is zebrafish brain.

Thanks

Best Regards

Use the same sample as negative control. But after you have incubated it with the primary antibody, just do not add the second antibody. Add PBS instead. It should work fine.

Regards,

Shafiq

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