Linearization of pPic9 plasmid using SacI?

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Installing Climate Data Operators?

Hello, I am trying to run Cygwin terminal but I experience a problem of cyproj-13.dll not found as below. I even checked on Cygwin but it is not there. Kindly help.

11 June 2024 4,502 0 View

How do I publish my research work here?

I need information on this question

21 May 2024 1,285 1 View

I design a unit cell metamatarial but the array structure does not behave like the unit cell, what could be the possible problem?

I created a unit cell in CST, and after that I created array from the structure, but my array structures do not behave like the unit cell, a have a DNG material in the unit cell and a SNG in the...

17 May 2024 4,700 6 View

Is there a glucose-free MEM-alpha medium commercially available?

For an assay I am planning to run I require a glucose-free MEM-alpha medium, I've seen papers where researchers state they use glucose-free MEM-alpha but they do not provide a supplier or catalog...

02 May 2024 4,004 0 View

IPSC to motor neurons?

Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...

01 May 2024 3,047 0 View

Clarification on calculating LogID50?

Can someone please clarify the correct way to calculate LogID50 using the Reed and Muench method. Using the example values below: 10^-1 100% positive 10^-2 100% positive 10^-3 100% positive 10^-4...

01 May 2024 4,113 0 View

Does the alkyl chain between the carbonyl group and the C=C bond convert this group to saturated?

Find attached file

30 April 2024 6,886 0 View

What is the physical reason that hydroponic plants can be planted closer together then soil-based plants?

In other words, is it simply the nutrient supply that makes soil plants much less effective when they are closer together or does it have to do with the roots interfering with each other. If it's...

16 April 2024 6,718 4 View

Does the alkyl chain decrease the impact of the ring on C=O stretching IR?

Find the attached file.

15 April 2024 6,749 1 View

No cytokine or complement activation in my PBMC stimulation assay?

Hi I`m trying to detect immune response in vitro with my nanoparticle (virus-like particles) incorporating SARS-CoV-2 Spike protein. I inoculated quite a lot of nanoparticle in PBMC and inoculated...

09 April 2024 173 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,572 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,056 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,458 5 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,891 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,647 1 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,482 5 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,228 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,556 1 View