HEK-293T cell line can be used as normal cell line if i am investigating the anticancer potential of a compound against a panel of cancerous cell line (considering HEK-293T as normal among the panel)?
HEK-293 is normal human cell line. Just put in your mind that it is a transformed by adding the SV40 vector. So if the anticancer agent is interfering with the SV40, it may be not the ideal. if you are using breast cancer cell line, then ideally use Hs578T (cancerous) and Hs578Bst (non Cancerous but derived from the same patient).