Transfection reagent for HEK-293T ?

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Installing Climate Data Operators?

Hello, I am trying to run Cygwin terminal but I experience a problem of cyproj-13.dll not found as below. I even checked on Cygwin but it is not there. Kindly help.

11 June 2024 4,502 0 View

How do I publish my research work here?

I need information on this question

21 May 2024 1,285 1 View

I design a unit cell metamatarial but the array structure does not behave like the unit cell, what could be the possible problem?

I created a unit cell in CST, and after that I created array from the structure, but my array structures do not behave like the unit cell, a have a DNG material in the unit cell and a SNG in the...

17 May 2024 4,700 6 View

Is there a glucose-free MEM-alpha medium commercially available?

For an assay I am planning to run I require a glucose-free MEM-alpha medium, I've seen papers where researchers state they use glucose-free MEM-alpha but they do not provide a supplier or catalog...

02 May 2024 4,004 0 View

IPSC to motor neurons?

Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...

01 May 2024 3,047 0 View

Clarification on calculating LogID50?

Can someone please clarify the correct way to calculate LogID50 using the Reed and Muench method. Using the example values below: 10^-1 100% positive 10^-2 100% positive 10^-3 100% positive 10^-4...

01 May 2024 4,113 0 View

Does the alkyl chain between the carbonyl group and the C=C bond convert this group to saturated?

Find attached file

30 April 2024 6,886 0 View

What is the physical reason that hydroponic plants can be planted closer together then soil-based plants?

In other words, is it simply the nutrient supply that makes soil plants much less effective when they are closer together or does it have to do with the roots interfering with each other. If it's...

16 April 2024 6,718 4 View

Does the alkyl chain decrease the impact of the ring on C=O stretching IR?

Find the attached file.

15 April 2024 6,749 1 View

No cytokine or complement activation in my PBMC stimulation assay?

Hi I`m trying to detect immune response in vitro with my nanoparticle (virus-like particles) incorporating SARS-CoV-2 Spike protein. I inoculated quite a lot of nanoparticle in PBMC and inoculated...

09 April 2024 173 2 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,707 1 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,018 3 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...

02 August 2024 7,842 0 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,571 0 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,025 3 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View