Morpholinos and Zebrafish?

More Thomson Patrick Joseph's questions See All

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem?

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem.

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How to perform Haldane correction on SPSS?

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Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

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07 February 2021 3,880 9 View

How to analyze dendritic spines from tissue (Prelimbic cortex)?

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27 January 2021 9,251 2 View

High throughput screen to identify drug induced cell death mechanism?

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How can I carry out analysis of pulp and paper properties according to Tappi standard method in the Lab?

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PROCESS V3.5.2 macro for SPSS variable name length limitations?

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What is the kinetics that Cr6 on the surface of the soil will preferably exchange with the charge on the surface of the iron oxide nanoparticle?

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Can anyone direct me to where I can find the X-Ray elastic constants for WC-Co or just WC?

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Anyone know of a good progesterone antibody?

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Which polarizers are usually used to measure the birefringence of zebrafish muscles?

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Is there any free software for behaviour analysis of zebrafish ?

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16 February 2021 1,541 1 View

Has anyone used a premade hybridization buffer (from Roche for example) for zebrafish in situ hybridizations?

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Can any tell me or suggest any research article or review about the concentration of peptide dissolve in system water and given to Adult Zebrafish?

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01 February 2021 5,809 3 View

Has anybody a protocol to determine the AChE activity in larvae zebrafish brain?

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14 January 2021 385 2 View

Anti-PCNA antibody [PC10] to detect proliferating cells in the brain of adult zebrafish (sections)?

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Best method to detect cell death in the brain of adult zebrafish on sections?

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How many times should I inject the drug before I take samples for RNA-Sequencing?

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08 December 2020 4,643 2 View

Anybody tried anti-Ki67 antibody to detect cell proliferation in adult zebrafish?

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Sandeep Paul

Hi Thomson,

Short answer: yes, however there are a number of caveats.

Long Answer: It depends. You would have to characterize both MOs individually so they give a specific phenotype, perform all proper controls, and then you can co-inject those MOs. Look at the primer published by Didier Stainier and colleagues (Article Guidelines for morpholino use in zebrafish

). We have stopped using MOs since 2012 and make CRISPR mutants. It may take a bit more time but it much more reliable way to assess LOF.

Thomson Patrick Joseph

Hello...manh thanks sandeep...

Jacek Topczewski

Yes this is standard technique.

Many thanks Jacek Topczewski