Nucleotide length between the two loxP sites?

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Installing Climate Data Operators?

Hello, I am trying to run Cygwin terminal but I experience a problem of cyproj-13.dll not found as below. I even checked on Cygwin but it is not there. Kindly help.

11 June 2024 4,502 0 View

How do I publish my research work here?

I need information on this question

21 May 2024 1,285 1 View

I design a unit cell metamatarial but the array structure does not behave like the unit cell, what could be the possible problem?

I created a unit cell in CST, and after that I created array from the structure, but my array structures do not behave like the unit cell, a have a DNG material in the unit cell and a SNG in the...

17 May 2024 4,700 6 View

Is there a glucose-free MEM-alpha medium commercially available?

For an assay I am planning to run I require a glucose-free MEM-alpha medium, I've seen papers where researchers state they use glucose-free MEM-alpha but they do not provide a supplier or catalog...

02 May 2024 4,004 0 View

IPSC to motor neurons?

Hi I have following questions regarding iPSC-derived motor neurons: 1. Is it possible to dettached mature motor neurons using accutase and reattach them on coated plates (matrigel, geltrx etc)?...

01 May 2024 3,047 0 View

Clarification on calculating LogID50?

Can someone please clarify the correct way to calculate LogID50 using the Reed and Muench method. Using the example values below: 10^-1 100% positive 10^-2 100% positive 10^-3 100% positive 10^-4...

01 May 2024 4,113 0 View

Does the alkyl chain between the carbonyl group and the C=C bond convert this group to saturated?

Find attached file

30 April 2024 6,886 0 View

What is the physical reason that hydroponic plants can be planted closer together then soil-based plants?

In other words, is it simply the nutrient supply that makes soil plants much less effective when they are closer together or does it have to do with the roots interfering with each other. If it's...

16 April 2024 6,718 4 View

Does the alkyl chain decrease the impact of the ring on C=O stretching IR?

Find the attached file.

15 April 2024 6,749 1 View

No cytokine or complement activation in my PBMC stimulation assay?

Hi I`m trying to detect immune response in vitro with my nanoparticle (virus-like particles) incorporating SARS-CoV-2 Spike protein. I inoculated quite a lot of nanoparticle in PBMC and inoculated...

09 April 2024 173 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,136 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,056 3 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

How can we identify (in silico) the interacting amino acid residues or the nucleotides involved in the Protein-Protein / Protein-RNA interaction?

Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...

14 July 2024 950 2 View

Deletion of Nucleotides in Phosphorylated Primer?

I was performing a site directed mutagenesis of a plasmid, the goal was to change/insert 4 nucleotides into the original plasmid using primers as seen in the image: Now I now there may be some...

04 July 2024 5,220 2 View

Anybody with experience with whole plasmid sequencing? Should I worry about plasmid oligomers?

I've sent my plasmids for whole plasmid sequencing using the Oxford nanopore sequencing method. All of them were assembled as monomers, but in the reads (see attached pictures), there are even...

19 June 2024 1,278 5 View

Why no gene deletion in S. cerevisiae using Lox p system?

Lox p was integrated at correct location, but unable to express cre recombinase to delete gene. Performed at different log hour and different Galactose concentration. Even though no gene deletion...

17 June 2024 1,068 3 View

Can anyone explain why my EGFP expression increased (but Ai9 RFP decreased -like normal) after imaging with a confocal microscope?

I have been trying to image a receptor in the brain for weeks now. It is an EGFP-cre G-coupled protein receptor. The model is a iCre based EGFP KI mouse model crossed with an Ai9 reporter line. I...

23 May 2024 7,773 2 View

Cre-loxP mating doesn't lead to pups with both alleles, any suggestions?

We crossed SynCre mice with Tg403-loxp-rtTA mice, but all the pups ended up with either one of the genes, not both together, as confirmed by genotyping tests. Has this ever happened to anyone else?

15 May 2024 3,964 2 View

Why do u get specific nucleotide contamination on sanger ?

We are

12 May 2024 1,667 1 View