X-Gal staining was performed. ... and samples of long bones were fixed, decalcified and processed for frozen section preparation followed by X-Gal staining.

Efficient Cre-recombination achieved in adult articular chondrocytes 1 and 6 months after TM induction

http://www.oarsijournal.com/article/S1063-4584%2807%2900279-8/abstract?cc=y?cc=y

I did X-gal staining first and decalcify bone tissue. seems OK. you do need to worry about it.

Dear Krishnan &

Dear Liu,

Thanks, for sharing this very critical experience with me, I appreciate,

Best,

Subhash

What sort of X-Gal staining are you doing?  Is it for aging, a reporter or to validate recombination?  It can be a bit tricky andI have found better results are obtained by staining with a Life Technologies antibody for the later two cases.

Thanks, Andrew,

For reporter,

Subhash