Anyone wants to share their experience of MSCs differentiation into articular cartilage and growth plate cartilage, and exact growth factors used for either type, i.e, Tgb and Bmp
To differentiate MSC in articular chondrocyte using micropellet, you might use both BMP2 or TGF-b3.
Formation of cartilage essentially
requires the presence of TGFβ, alone or in combination
with BMP2 or GDF5. The kinetics of gene
expression during chondrogenesis suggest that
this differentiation program is initiated faster in
chondrogenic pellets treated with TGFβ in combination
with BMP2 than with TGFβ alone or TGFβ
in combination with GDF5.
https://www.researchgate.net/publication/235983639_Gene_expression_regulation_underlying_Osteo-_Adipo-_and_Chondrogenic_lineage_commitment_of_human_mesenchymal_stem_cells
Chapter Gene Expression Regulation underlying Osteo-, Adipo-, and Ch...
Thanks. Stephane and Ana Maria,
I am trying to distinguish hypertrophic vs articular cartilage. which factors for either.
Thanks,
Subhash
Dear Dr. Subash,
TGF-beta-3 is the key factor promoting the differentiation of MSCs to cartilage either by mono-layer,micro-mass or pellet culture systems.With the little experience i have,i would believe that the number of days defines articular cartilage and hypertrophy.Extended differentiation would up-regulate the hypertrophic marker ColX. Essentially 12-16 days of differentiation would result in healthy articular cartilage.Extending it for another 10-15 days will result in hypertrophy.
Hope this helps!
Regards,
Charan
Stempeutics Research
Thanks, Charan, I will look carefully, I made some cartilage on collagen coated inserts at 6-wk, they were more towards hypertrophic side, will look at different and early days,
Best,
Subhash