33 Questions 41 Answers 0 Followers
Questions related from Shahina Akter
Hello I am currently engaged in research focusing on plant genetic transformation. As part of this endeavor, I have designed a comprehensive in silico plasmid cloning approach. The aim is to...
08 May 2024 5,080 3 View
Hello, I hope this message finds you well. I am currently planning to synthesize a long chain oligonucleotide using our oligonucleotide synthesizer. However, I find myself uncertain about the...
08 May 2024 7,406 1 View
Hi Dear Scientists I am currently working on an exciting project involving edible vaccines, and I wanted to seek your guidance regarding the next steps in my research. To begin with, I have...
20 July 2023 427 4 View
Hi, dear fellow scientists! I am facing a problem while trying to construct a plasmid vector for developing a vaccine. After immunoinformatics studies, I have designed a plasmid vector. Now I...
12 April 2023 9,519 4 View
Hi From the human whole-genome sequenced data how can I analyze the gene? I can filter one gene manually, but how about a gene pool??? How do I interpret the annotated data from vcf file? As a...
22 May 2022 8,529 0 View
Dear Researchers I have completed two manuscripts on SARS-CoV-2. Unfortunately, I do not have any grant or any other source of funds that can pay the APC. In this circumstance, I want to submit...
20 March 2022 3,645 5 View
Dear scientists Our team sequenced the human whole-genomes for breast cancer genes analysis. We could manage to generate up to the VCF files. Now we want to annotate the data and do the KEGG...
03 March 2022 1,313 3 View
I have performed human whole-genome sequencing using NovaSeq6000 and analyzed the data using Basespace. Now I got some results for breast cancer 28 genes list which has been assembled in an excel...
15 December 2021 2,538 1 View
I need to identify a bacteria from my culture. For that reason, I sequenced the bacteria and it is showing that Agrobacteria fabrum. These two bacteria are same or different?
07 December 2021 2,034 4 View
I have sequenced my Bacteria using NGS and got fastq data. For analysis now I need to convert this data into FASTA. How can I do that easily? Should I use galaxy or recommend any other good tool?...
05 December 2021 6,049 7 View
I want to do plant genetic transformation with my gene of interest. For that reason, I need to construct the vector Ti plasmid so that can go for Agrobacterium-mediated plant genetic...
01 December 2021 8,132 2 View
I have some experience in Human, SARS-CoV-2, Dengue and whole Genome Sequencing, and bacterial metagenomics. Now I want to do some work on plant genomics. Can anyone share some knowledge regarding...
14 September 2021 1,469 7 View
We have sequenced SARS-CoV-2 and submitted it to the GISAID. From there how I can find out the unique mutations? There are thousands of mutations showing in the table, from there how I understand...
01 August 2021 5,967 3 View
After inoculation on Citrimide Agar I got such colonies. Can anyone identify these bacteria or fungi?
20 June 2021 8,865 3 View
We have sequenced over 600 SARS-CoV-2 whole genomes of Bangladeshi Patients. Now for analyses need to make the phylogenetic tree. Before we did it using Nexstrain, this tool is not suitable as it...
25 April 2021 4,741 5 View
Hello Researchers, I would like to annotate my FASTA sequence of COVID19 of many samples. I aligned the data using MegaX and analyse spike protein using Swiss-model. I could not interpret...
25 March 2021 680 4 View
Any kind of drug that inhibits the growth and infection of any kind of bacteria in the human body so that the mode of infection will be changed and person keep safe from the bacteria especially...
24 March 2021 1,827 2 View
I want to know about the current status of plant genome sequencing in this world. How is the reference genome in this case? Does anybody have any knowledge regarding the topic of Plant genome...
23 March 2021 3,020 5 View
Hi Greetings to all of you. We have sequenced SARS-CoV2 whole genome sequence using NextSeq and with the fasta files, I managed to translate the file into protein sequence. I have aligned those...
31 December 2020 5,132 3 View
Hi I got a 3D protein structures in my research and want to superimpose it with the wild. I am working with SARS-CoV2 and found a deletion that I want to compare with that of Wuhan as a wild. I...
17 December 2020 4,053 5 View
I have some sequences of Spike protein of some Covid 19 samples that have been sequenced in my laboratory using NGS. For analyses of those, I need to convert those sequences into PDB format. I am...
02 December 2020 822 3 View
After doing alignment of some protein sequences using Clustal Omega, I got the result that I have been attached herewith. I am seeking exparts opinion so that can find a good explanation of the...
24 November 2020 1,780 6 View
I have sequenced many covid 19 samples and now I want to separate only the spike protein portions from the samples of covid 19 positive patients so that I can do a model of the protein. Thanking...
04 November 2020 2,695 4 View
After doing a PCR with my specific primer I got multiple bands instead of getting a unique band. I am totally astonished after getting this. Can anyone guessed what happened why I get this band...
27 October 2020 3,905 17 View
Hi We have sequenced more then 50 samples of Covid-19 patients and submitted to GISAID. There I got some unique mutations which are absent in mutation statistics of GISAID CoVsurver. The mutations...
23 June 2020 1,879 7 View
Usually we know that Streptococcus pneumoniae affects people in cold. But while I tried to culture it the temp. needs 37C in the incubator with CO2. Why this temparature variable anybody can answare?
12 March 2020 4,903 3 View
How can I create gene specific bed file from whole genome sequencing data using command line or using software? In case of software what will be the pipeline. We are able to generate bam file and...
04 December 2019 4,276 0 View
I tried to isolate plasmid DNA from Agrobacterium tumefaciens using both manually and using kit and performed the electrophoresis on 0.5%, 0.8% and 1.0% agarose gel. The ladder showed the...
29 September 2019 6,565 2 View
For the research of Agrobacterium mediated genetic transformation I need to construct plasmid vector. If I design that vector using a suitable software, after that how I could make the construct....
16 April 2018 1,025 3 View
I am using media containing BHI with FBS for liquid culture and Blood agar plate for subculture of Streptococcus pneumoniae. From this how can I stock it? What will be the suitable media and...
21 August 2017 4,019 3 View
Dear Researchers Nowadays we people of this world are now in a very hard time as SARS-CoV-2 affects us a lot. Some vaccines we know already have released even those that are not accurately...
01 January 1970 162 20 View
Hi Dear Scientists I have completed work and prepared a manuscript on immunoinformatics. As I do not have such funds to pay the APC, and as I am from a least-developed country, I am searching for...
01 January 1970 7,513 2 View
We have performed human whole-genome sequencing of 100 Bangladeshi samples using NovaSeq6000 and analyzed the data using Basespace. Now I got some results for breast cancer 28 genes list along...
01 January 1970 5,131 1 View
