For the research of Agrobacterium mediated genetic transformation I need to construct plasmid vector.
If I design that vector using a suitable software, after that how I could make the construct.
Or anybody can co-operate me in this aspect by providing the construct according to my need? That will be a collaborative work.
Hi Akter, my professor called this cloning by telephone: Addgene is a non commercial place for sharing vectors. Please check this webpage and look if there is already a vector clones which could fit to your application:
https://www.addgene.org/
There are lots of ways in which you could make the construct. If you already have your genes of intrest in some other constructs, you can just PCR out the fragments and proceed with cloning. The cloning/vector design software can help you with primer design. In case you do not have any starting material available, you can get your construct commercially synthesized according to your specifications.
Thank for the answer.
Do you know some of the company or organisation can help to make a construct?