Hi, dear fellow scientists!
I am facing a problem while trying to construct a plasmid vector for developing a vaccine.
After immunoinformatics studies, I have designed a plasmid vector.
Now I intend to construct that particular plasmid vector.
I intend to order a commercially available empty plasmid for this purpose. My question is, How can I get the designed vaccine that I have made after molecular docking and simulation? How can I make that particular protein ligate into the plasmid?
Based on the amino acid sequence of your peptide you can infer the codons needed and get the sequence chemically synthesized (eg. IDT or Eurofins). you then can clone that sequence into your plasmid (or subclone via a TOPO vector).
If you are looking to construct a plasmid vector containing a designed protein or vaccine, you will likely need to use a cloning strategy in order to insert the designed gene into the plasmid vector. In order to do this, you will need to use PCR to amplify the gene of interest and then use restriction enzymes to cut the vector and insert the gene. Once the gene is inserted, you can then use transformation to introduce the plasmid vector into bacterial cells. After the plasmid is replicated in the cell, it can be isolated and used for further experimentation.
Snapgene software will help you designed appropriate plasmids with insert (your designed vaccine sequence). Take a look at the software.
Good luck
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