I have sequenced many covid 19 samples and now I want to separate only the spike protein portions from the samples of covid 19 positive patients so that I can do a model of the protein.
Shahina Akter You can amplify your gene by PCR. That means you have to order specific primers, you can search for primer sequences in literature - eg like thisArticle Primer design for quantitative real-time PCR for the emergin...
after you have obtained the gene, it must be inserted in a plasmid for protein expression.
As Alina Lenghel mentioned, you need to amplify the gene sequence of spike protein from your sample, and for that you need to read the literature and design a pair of specific primers.