I am using media containing BHI with FBS for liquid culture and Blood agar plate for subculture of Streptococcus pneumoniae. From this how can I stock it? What will be the suitable media and protocol?
I maintained Streptococcus pneumoniae in 20% glycerol (v/v in cation-adjusted Mueller Hinton broth with 10% lysed human blood v/v in sterile distilled water) at −20 °C.