In natural sources what is the purity of L-DOPA and does that purity remain same in callus or cell culture?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
Hi all. I ran the enzyme activity (pure Tyrosinase after purification using recombinant e coli) using L-Dopa with this condition 50 mM phosphate buffer ph 6, 1mM L-Dopa 25 C temperature 0.5 mM...
06 February 2024 8,350 4 View
Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...
24 December 2023 9,368 1 View
We are currently having some problems with DAF2 or DAF-FM fluorescence. We use a 5µM concentration and are not able to see any effect of a stimulation on DAF fluoresnce. When looking cells under...
16 November 2023 8,182 0 View
If yes, what is the ideal concentration of L-Arginine in DMEM?
04 October 2023 1,856 0 View
I am using a magnetic separation based isolation kit for mouse neutrophils and the protocol mentions 2 incubation on ice for 15 min each. I am aware that low temperature pre-activate the...
05 September 2023 6,293 0 View
Greetings everyone. During my master's research, I focused on exploring the potential of a specific bacterial strain to produce antibacterial compounds. To achieve this, I used the technique of...
24 August 2023 588 4 View
it means we have a set protocol of using poly L lysine for coverslip coating, according to our protocol after 4 our of incubation in 37 C ,we are used to dicard the poly L lysine ,but as the poly...
09 July 2023 5,335 0 View
I have L-Lysine powder from Sigma Aldrich. Based on MSDS, the L-Lysine powder can easily be solved with water, but in my case, some hydrophobic parts can't be solved. It influences the UV-Vis...
12 June 2023 4,664 5 View
Hi every body I made the ship's environment based on M9 culture media with the following ingredients( per 100 ml culture media ): KCL= 0.2 gr NaCl= 0.01 gr KH2PO4 =0.2 gr MgSO4= 0.001 gr Fe so4...
16 May 2023 8,115 0 View
In the article titled "FDOPA-(18F): a PET radiopharmaceutical recently registered for diagnostic use in countries of the European Union", two half-lives are reported for FDOPA....
19 November 2022 7,799 9 View