29 Questions 48 Answers 0 Followers
Questions related from Milad Esmaeilbeigi
I need an applicable molecular biomarker like expressing the genes in frogs who expose to chytrid fungus in different environmental stressors. Indeed, what kinds of genes I should notice in frog's...
26 January 2021 8,094 2 View
I have many hands-on experiences in carrying out Comet Assay method on various animal cells, but I haven't had any background to investigate DNA damage in aquatic plants so far. As they are...
16 January 2021 5,073 2 View
I'm looking for the cause of this disorder.
31 August 2015 5,745 4 View
i mean percentage of protein, lipid, carbohydrate and other additives.
24 July 2015 7,981 1 View
the images is from gill and liver tissue in fish that expose to benzo(a)pyrene(oil contaminant).
03 June 2015 5,785 4 View
five comet index includes:comet lenght, comet heigth, tail lenght, %DNA in tail and tail moment i have five treatment: control, solvent(negative control), 50ppb, 100ppb and 200ppb(concentration of...
03 June 2015 3,289 2 View
i mean are chemical compounds of lysis buffer able to damage, probably. lysis solution include: (2.5 M NaCl, 0.1 M EDTA, 10 mM Tris, 1% Triton X-100, 9% DMSO, pH 10)
17 May 2015 2,137 4 View
i need information about making new diet like biomar for koi or goldfish
11 May 2015 4,598 1 View
I mean: after what time from start exposure, DNA damage is started and after what time, repair is started?
03 February 2015 4,530 5 View
if possible, attaching a file for this method please.
29 January 2015 6,828 3 View
what is the best condition for freezing and defreezing of samples at -80 freezer?
19 January 2015 2,966 1 View
In fact, I want to dissolve organic selenium for preparation stock, then spray this stock on food and feeding goldfish with formulated diet.
15 January 2015 3,168 2 View
i want cutting the gill and liver tissue of fish
12 January 2015 982 13 View
I would like to prepare a cell suspension from liver and gill tissue.
10 January 2015 1,822 2 View
As well as what is the best solvent for it? I am carry out liver and gill cells of fish
09 January 2015 1,759 6 View
i used 10 microliter ethidium bromide for each loaded samples(two) on slide(i.e 20 microliter for each slide) but under the fleurosent microscope, can hardly see the halo DNA or comet. One of the...
07 January 2015 5,782 3 View
the statistical test that can be relationship beetwen frequency of micronucleuses and cocentration and period exposure.
07 January 2015 2,717 13 View
i.e should lysis buffer be prepared fresh and daily or it can be used for many times؟
07 January 2015 1,087 5 View
in fact I want using gill and liver cells.
07 January 2015 1,466 2 View
Micronucleus assay to be carried out on fish. I am attaching several images, Please tell us your opinion.
05 January 2015 4,039 4 View
steps my work in the process of cell suspension:1- after the exit microtube(from freezer -80C) containing liver tissues and PBS buffer, Samples are placed on ice until the ice melted2- then...
05 January 2015 5,674 6 View
I want to prepare cell suspension from liver and gill tissue and coated on slides.
30 December 2014 7,400 1 View
in slides under microscope, only parts of the tissue or other things are found and the comet tail is not found.
30 December 2014 9,323 3 View
MNscore is a good software for automaticaly calculation of micronucleus frequency.
30 December 2014 7,692 2 View
In fact, I want informations about fish (preferably).
28 December 2014 4,898 10 View
In fact, I am searching for a program capable of analyzing all comets on a slide at once.
25 December 2014 8,751 4 View
as regards that etidum bromide stain is very sensitive to light
25 December 2014 856 4 View
In fact, I am searching for a program capable of analyzing all comets on a slide at once!
24 December 2014 8,391 4 View
Aquatic animals as a highly sensitive group of organisms in both of marine and freshwater ecosystems encounter a variety range of endocrine disruptors. Estrogen like compounds;for example, in...
01 January 1970 1,102 6 View