What is a suitable way to obtain large numbers of cells for a comet assay?

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Temitope O. Sogbanmu

Hi.

I'm not sure if there's a 'best way'.

What I did and which I suggest you try, is to work with different dilutions of your cell suspension. You could reduce the dilution factor of your cell suspension with the Agarose before coating on the slide to obtain more cells.

It might also be that the amount of your tissue you're using is not enough or you're losing cells during the preparation of your tissue for comet assay.

Hope this helps

Milad Esmaeilbeigi

thanks temitope

after cutting tissues(completely) by scissors, Solution produced in microtube was released for ten minute, then 10 microliters from above cell suspension is removed and mixed by LMPA and loaded on slides.

Do you agree with procedure?