What is the best method for doing comet assay test on fish sperm?

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Can anyone recommend software for creating scientific illustrations (or charts, graphs, etc.)?

I'm looking for something user-friendly with good data visualization tools. already tried OriginLab and GIMP.

04 June 2024 945 3 View

Which ubuntu version is fully compatible with ASUS Prime Z790M-Plus motherboard?

Dear Expert I have a PC containing ASUS Prime Z790M-Plus motherboard and Intel 13th gen CPU and need to install ubuntu on it. I have searched this list...

19 March 2024 5,160 2 View

High Power Inductor and reactor design?

hi, I need a practical calculation and design for designing passive harmonic filter inductors. and the information about core sizing and considerations for 3-phase system.

09 February 2024 1,913 1 View

Is there any protocol for the histological evaluation of colon biopsies after ex-vivo culture?

I want to evaluate and compare human colon biopsies after 24h ex-vivo treatment with inflammatory and non-inflammatory cytokines. Apart from the H&E assessment, is there any protocol for the...

08 February 2024 5,357 3 View

Is synergetic control a model-free or model-based approach?

Is synergetic control a model-free or model-based approach? Please tell me the reasons. How about PID control?

07 February 2024 6,823 2 View

What are the origin of crystals that form upon heating castor oil?

In the process of maleinizing castor oil with maleic anhydride I am observing formation of crystal-like structures upon heating the solution above 100 degrees Celsius. The solution is just castor...

02 February 2024 5,095 1 View

Hello, I need heat loss and heat combustion formulas and equations for two-stroke diesel engine?

In the case of two-stroke diesel engine high pressure and temperature happen in combustion stage which releases Q_combustion and Q_loss. So I look for these two parameters formuals and equations...

11 January 2024 5,206 0 View

Difference between output power variations of (Pd - coated) and (Pd-Cu -coated) tapered optical fiber?

Output power changes on the optical fiber sensor for hydrogen gas have been illustrated. Why does the Pd-Cu sensor show no sensitivity at low hydrogen percentages while the Pd sensor does? Why...

01 January 2024 2,200 2 View

Change in multiple band gap of CuSe after Ni-doping?

The band gap results for CuSe and Ni-doped CuSe, according to the Tauc plot and the mathematical DITM (Derivation Ineffective Thickness Method) model, in which thickness and type of transition...

29 December 2023 614 2 View

"Optimizing Electrochemical Sensors: Molecular Imprinting Polymer Technique and AutoDock Vina Analysis of Functional Monomers"?

In the development of electrochemical sensors utilizing the Molecular Imprinting Polymer (MIP) technique, I retrieved functional monomers from existing literature. Can AutoDock Vina be utilized to...

22 December 2023 3,955 1 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View