I have many hands-on experiences in carrying out Comet Assay method on various animal cells, but I haven't had any background to investigate DNA damage in aquatic plants so far. As they are single cells in their populations, it seems to me such cells possess a merit to conduct DNA damage studies (single cell gel electrophoresis). However, I don't know how I can conduct an Alkaline Comet Assay procedure on either marine or freshwater microalgae? especially when I need to remove a plant cell wall which is more harder than the animal one (lysis step) .

I really appreciate it if you attach instructions and related papers.

Many thanks

Milad

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