Dear Milad,

Thanks for your nice Histopathology Images. It seems informative and usefull for all pathologist. As you know benzo(a)pyrene could be a hazard carcinogen and could be occur a kind of Spontaneous liver tumors in mice in experimental trial.

Globing and Clubbing in second Gill lamellae, Atelectasis and some inflammation effects that lead to spread necrosis in liver could be more important lesions in your Images,

Also, defects in the basement membrane, enlarged nuclei with cytoplasmic invaginations, and pleomorphic nucleoli were restricted to squamous metaplasia induced by benzo[a]pyrene-ferric oxide. 

Finally if you are interesting for more share information about above subjects please correspond with me through my email as follows:

[email protected]

I can prepare more information for your article in this regard.

Yours truly

Jalil

Dear Milad,

My presumptive diagnosis:

epithelial lifting (fig. 5)

club shaping of gill lamellae (fig. 2)

Dilation of marginal vascular channels (figs. 1 and 2)

nuclear degeneration and cytoplasmic degeneration (figs. 6 and 7)

cytoplasmic vacuolation (all figs. liver tissue)

Best wishes,

Ebrahimzadeh

Dear Respected sir

                        My Project work Fish toxicology -

                                    Tissues damages  

       Gill     - fish epithelial membrne- respiratory problem

       Liver    -Hepatoprotective cells Defected-cells decrease and cells collaboration

    Muscle - normal and abnormal arrange bundle

  All tissues observed your treatment Period then you mentioned to your chemical name  

                                Thanking you

First of all I would like to compare these pictures with the pictures of control fish.Did you find  differences between the control and the experemental fish?