five comet index includes:comet lenght, comet heigth, tail lenght, %DNA in tail and tail moment

i have five treatment: control, solvent(negative control), 50ppb, 100ppb and 200ppb(concentration of benzo(alfa)pyrene, oil contaminant) and each treatment contain  3 replicate

expose period: 21 day

sampling times: 0, 2, 7, 14 and 21 days from two tissue of fish:gill and liver.