How can I prepare a cell suspension from frozen tissues (liver and gill) of fish?

19 January 2015 1 3K Report

what is the best condition for freezing and defreezing of samples at -80 freezer?

Hi Milad

firstly you have to thawing the tissues step by step to prevent cell wall distraction. in this order the time of defreezing would be longer than usual and temperature should increase degree by degree.

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Science method

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