I got the best results with 0.75% LMPA in destilated water (higher concentration of LMPA will interfere with migration of high molecular weight DNA towards the anode and the pictures at the end of analysis will be blurred). 

I used to get good results with 0.5% LMPA in PBS.

I made a mistake - I used PBS not water

Hi.

I got good results with 0.75% LMPA dissolved in PBS.

i coated the slides with NMA ,cooled and dried, and then applied blood cells plus LMP agar in distilled water.Igot results but the results were much better with Trivegen Kit

Mr. Milad,

Use of 0.5% LMPA in PBS. Standardize cell concentration for after trial for good comet slides. 

Good luck!